小鼠肝脏S-腺苷甲硫氨酸合成酶信使核糖核酸的体外翻译及调控

Translation in vitro and regulation of mouse liver S-adenosylmethionine synthetase messenger RNA.

作者信息

Sato F, Kitahara N, Ohmura N, Tsukada K

出版信息

FEBS Lett. 1983 Jan 10;151(1):148-52. doi: 10.1016/0014-5793(83)80361-5.

Abstract

Total RNA was isolated from adult mouse liver tissues. The alpha- and beta-form isozymes of S-adenosylmethionine synthetase existing in liver were synthesized in a reticulocyte lysate cell-free system under the direction of total RNA and were immunoprecipitated with antibody to the beta-form. The newly synthesized and the in vivo labeled S-adenosylmethionine synthetase subunits were compared by SDS-polyacrylamide gel electrophoresis. Both the alpha- and beta-forms consist of the same size Mr 48 000 subunit. The level of the beta-form mRNA activity in mouse liver was shown to increase following intraperitoneal transplantation of Ehrlich ascites tumor cells and the changes in the mRNA activity parallel those in the cellular level of S-adenosylmethionine synthetase beta.

摘要

从成年小鼠肝脏组织中分离出总RNA。在无细胞的网织红细胞裂解物系统中，在总RNA的指导下合成肝脏中存在的S-腺苷甲硫氨酸合成酶的α和β形式同工酶，并用抗β形式的抗体进行免疫沉淀。通过SDS-聚丙烯酰胺凝胶电泳比较新合成的和体内标记的S-腺苷甲硫氨酸合成酶亚基。α和β形式均由相同大小的Mr 48 000亚基组成。结果显示，腹腔移植艾氏腹水瘤细胞后，小鼠肝脏中β形式mRNA的活性水平升高，且mRNA活性的变化与S-腺苷甲硫氨酸合成酶β的细胞水平变化平行。