Booth S C, Mould A J, Shaw A, Garner R C
Mutat Res. 1983 Feb;119(2):121-33. doi: 10.1016/0165-7992(83)90120-3.
4-Chloromethylbiphenyl (4CMB), benzyl chloride (BC) and 4-hydroxymethyl-biphenyl (4HMB) were tested for biological activity in the following assays: (i) the Salmonella/microsome assay; (ii) a bacterial 'fluctuation' assays; (iii) a DNA repair assay in Hela cells, and (iv) a mouse lymphoma mutation assay. 4CMB was active in assays (i), (ii) and (iii) but not in (iv); BC was active in assays (i), (ii), (iii) but not in (iv) while 4HMB was inactive in all assays. Where biological activity was seen this did not require addition of a liver S9 preparation. 4CMB was more active than BC in all the test systems in which a positive response was obtained. The implication of these results for a test battery approach to in vitro testing is discussed.
对4-氯甲基联苯（4CMB）、苄基氯（BC）和4-羟甲基联苯（4HMB）进行了以下生物活性检测：（i）沙门氏菌/微粒体试验；（ii）细菌“波动”试验；（iii）HeLa细胞中的DNA修复试验；以及（iv）小鼠淋巴瘤突变试验。4CMB在试验（i）、（ii）和（iii）中具有活性，但在（iv）中无活性；BC在试验（i）、（ii）、（iii）中具有活性，但在（iv）中无活性，而4HMB在所有试验中均无活性。在观察到生物活性的情况下，这并不需要添加肝脏S9制剂。在所有获得阳性反应的测试系统中，4CMB比BC更具活性。讨论了这些结果对体外测试组合方法的意义。
