Goldfine C, Miller W A, Haddow J E
Br J Obstet Gynaecol. 1983 Mar;90(3):238-40. doi: 10.1111/j.1471-0528.1983.tb08616.x.
Densitometry was used to measure gel acetyl- and pseudocholinesterase bands in a selection of amniotic fluid samples yielding positive alpha-fetoprotein and positive gel acetylcholinesterase results. The samples were associated with the following conditions: 28 anencephaly (10 stained with fetal blood), 20 open spina bifida (3 stained with fetal blood), 10 open ventral-wall defects (2 stained with fetal blood) and 5 unaffected pregnancies (all stained with fetal blood). In samples not visibly stained with fetal blood, the ratio of the acetylcholinesterase density to the pseudocholinesterase density distinguished between open ventral-wall defects and open neural-tube defects: the former group all had values less than 0.13, while the latter all had values greater than that. In amniotic fluid samples which were stained with fetal blood the acetylcholinesterase/pseudocholinesterase ratio did not offer useful diagnostic information; results from unaffected pregnancies overlapped with those from pregnancies with both ventral-wall defects and open neural-tube defects.
采用密度测定法，对一批羊水样本中凝胶乙酰胆碱酯酶和假性胆碱酯酶条带进行测量，这些样本的甲胎蛋白呈阳性，凝胶乙酰胆碱酯酶检测结果也呈阳性。这些样本与以下情况相关：28例无脑儿（其中10例被胎儿血染）、20例开放性脊柱裂（其中3例被胎儿血染）、10例开放性腹壁缺损（其中2例被胎儿血染）以及5例正常妊娠（均被胎儿血染）。在未被明显胎儿血染的样本中，乙酰胆碱酯酶密度与假性胆碱酯酶密度的比值可区分开放性腹壁缺损和开放性神经管缺损：前一组的所有值均小于0.13，而后一组的所有值均大于该值。在被胎儿血染的羊水样本中，乙酰胆碱酯酶/假性胆碱酯酶比值无法提供有用的诊断信息；正常妊娠的结果与腹壁缺损和开放性神经管缺损妊娠的结果相互重叠。
