Wald N J, Barlow R D, Cuckle H S, Turnbull A C, Goldfine C, Haddow J E
Br J Obstet Gynaecol. 1984 Sep;91(9):882-4. doi: 10.1111/j.1471-0528.1984.tb03701.x.
Amniotic fluid samples from eight pregnancies associated with exomphalos or gastroschisis and 24 with open spina bifida were used to confirm the hypothesis that when the alpha-fetoprotein and gel acetylcholinesterase tests are positive in an amniotic fluid not visibly contaminated with blood, the two defects can be distinguished by scanning densitometry of the electrophoretic gel. The ratio of the density for the acetylcholinesterase band in the gel to that of the pseudocholinesterase band was less than or equal to 0.10 for the open abdominal wall defects and 0.27 or more for open spina bifida. Based on this and a previously reported series, a cut-off level of about 0.15 is suggested.
采集了8例与脐膨出或腹裂相关妊娠以及24例开放性脊柱裂妊娠的羊水样本，以验证以下假说：当羊水甲胎蛋白和凝胶乙酰胆碱酯酶检测呈阳性且未明显被血液污染时，可通过电泳凝胶扫描光密度法区分这两种缺陷。对于开放性腹壁缺陷，凝胶中乙酰胆碱酯酶条带与假性胆碱酯酶条带的密度比小于或等于0.10，而对于开放性脊柱裂则为0.27或更高。基于此及之前报道的系列研究，建议临界值约为0.15。
