Royds J A, Rennie I G, Parsons M A, Timperley W R, Taylor C B
Br J Ophthalmol. 1983 Apr;67(4):244-8. doi: 10.1136/bjo.67.4.244.
Seven uveal melanomas were stained for gamma enolase by an immunoperoxidase PAP (peroxidase-antiperoxidase) technique, and biochemical assays were carried out on tissue homogenates. A correlation between the biochemical assay and the immunoperoxidase staining was demonstrated. Two tumours with the highest biochemical assays showed positive staining for the enzyme, and 2 tumours with the lowest levels showed no appreciable staining. The highest level of enolase was present in a tumour which both clinically and histologically appeared to be benign, and the lowest level occurred in a mixed cell tumour which was large in size; the low level presumably related to its relatively fast rate of growth. Estimation of gamma enolase activity in ocular melanomas may provide an accurate quantitative method for assessing the malignant potential of these tumours.
采用免疫过氧化物酶PAP（过氧化物酶-抗过氧化物酶）技术对7例葡萄膜黑色素瘤进行γ-烯醇化酶染色，并对组织匀浆进行生化分析。结果表明生化分析与免疫过氧化物酶染色之间存在相关性。生化分析值最高的2个肿瘤对该酶呈阳性染色，而最低水平的2个肿瘤未显示明显染色。烯醇化酶水平最高的肿瘤在临床和组织学上均表现为良性，而最低水平出现在一个体积较大的混合细胞肿瘤中；低水平可能与其相对较快的生长速度有关。评估眼黑色素瘤中的γ-烯醇化酶活性可能为评估这些肿瘤的恶性潜能提供一种准确的定量方法。
