Hildreth J E, Gotch F M, Hildreth P D, McMichael A J
Eur J Immunol. 1983 Mar;13(3):202-8. doi: 10.1002/eji.1830130305.
Human lymphocyte function antigen (HLFA) is a cell surface protein defined by two monoclonal antibodies MHM23 and MHM24. It is present on both B and T lymphocytes but in greater amounts on the latter. Both antibodies precipitated antigen, from radiolabeled HSB-2 cells, which ran as two chains on sodium dodecyl sulfate polyacrylamide gel electrophoresis at 180 and 94 kDa. Neither antibody inhibited binding of the other, indicating that distinct epitopes were recognized. Both antibodies were shown to inhibit HLA-restricted lysis of influenza virus-infected and Epstein-Barr virus-transformed target cells by cytotoxic T lymphocytes. Blocking occurred at the level of the effector cells and in the presence of subsaturating concentrations of antibody. Both reagents also inhibited lysis of K562 cells, mediated by natural killer cells. These blocking effects differ from the inhibitory effects of monoclonal anti-HLA ABC and anti-suppressor cytotoxic T cell antibodies which inhibit only HLA-restricted lysis when present in saturating amounts. It is concluded therefore that HLFA is likely to be involved in the nonspecific adherence or lytic functions of killer cells rather than specific antigen recognition.
人类淋巴细胞功能抗原（HLFA）是一种由两种单克隆抗体MHM23和MHM24所定义的细胞表面蛋白。它存在于B淋巴细胞和T淋巴细胞上，但在后者上的含量更高。两种抗体均可从放射性标记的HSB - 2细胞中沉淀出抗原，该抗原在十二烷基硫酸钠聚丙烯酰胺凝胶电泳上呈现为180 kDa和94 kDa的两条链。两种抗体都不会抑制另一种抗体的结合，这表明它们识别的是不同的表位。两种抗体均被证明可抑制细胞毒性T淋巴细胞对流感病毒感染的和爱泼斯坦 - 巴尔病毒转化的靶细胞的HLA限制裂解。阻断作用发生在效应细胞水平，且在抗体浓度未饱和的情况下。两种试剂还抑制了自然杀伤细胞介导的K562细胞的裂解。这些阻断作用不同于单克隆抗HLA ABC和抗抑制性细胞毒性T细胞抗体的抑制作用，后者只有在饱和量存在时才抑制HLA限制裂解。因此得出结论，HLFA可能参与杀伤细胞的非特异性黏附或裂解功能，而非特异性抗原识别。
