Clarke D W, Mudd L, Boyd F T, Fields M, Raizada M K
J Neurochem. 1986 Sep;47(3):831-6. doi: 10.1111/j.1471-4159.1986.tb00686.x.
Depolarization of neuronal cells in primary culture from the rat brain by potassium ions in the presence of calcium or by veratridine caused a greater than three-fold stimulation of release of immunoreactive insulin. HPLC of the released insulin immunoreactivity from the neuronal cultures comigrated with the two rat insulins. The depolarization-induced release of insulin was inhibited by cycloheximide and was specific for neuronal cultures since potassium ions failed to cause the release in comparably prepared astrocytic glial cells from the rat brain. Prelabelling of neuronal cultures with [3H]leucine followed by depolarization resulted in the release of radioactivity that immunoprecipitated with insulin antibody. The release of [3H]insulin was biphasic. These observations suggest that neuronal cells from the brain have the capacity to synthesize insulin that could be released under depolarization conditions.
在存在钙的情况下，钾离子或藜芦碱可使原代培养的大鼠脑神经元细胞去极化，导致免疫反应性胰岛素释放受到三倍以上的刺激。对神经元培养物中释放的胰岛素免疫反应性进行高效液相色谱分析，结果显示其与两种大鼠胰岛素共迁移。环已酰亚胺可抑制去极化诱导的胰岛素释放，这种释放对神经元培养物具有特异性，因为钾离子无法使同样制备的大鼠脑星形胶质细胞释放胰岛素。用[3H]亮氨酸对神经元培养物进行预标记，然后使其去极化，会导致放射性物质的释放，该放射性物质可被胰岛素抗体免疫沉淀。[3H]胰岛素的释放是双相的。这些观察结果表明，脑内的神经元细胞具有合成胰岛素的能力，且在去极化条件下可释放胰岛素。
