Wicha M S, Huard T K
Exp Cell Res. 1983 Feb;143(2):475-9. doi: 10.1016/0014-4827(83)90077-0.
Laminin, a non-collagenous extracellular connective tissue glycoprotein, was detected on the surface of mouse peritoneal macrophages. As determined by indirect immunofluorescence, as many as 60% of peritoneal macrophages elicited with thioglycollate expressed cell surface laminin. Only 14% of resident cells displayed detectable laminin. The expression of laminin increased with time post-injection. Concomitant with laminin expression, macrophages also displayed a receptor for the IB4 isolectin from Griffonia simplicifolia. This lectin, which binds methyl-alpha-D-galactopyranoside, may also react with the carbohydrate moeity of laminin. A small population of macrophages displayed both laminin and surface fibronectin. Unlike the difference in laminin expression between resident and thioglycollate-stimulated cells, there was no difference in cell surface fibronectin between these cell populations. Since laminin has been found to mediate cell attachment in other systems, expression of this molecule on the surface of stimulated macrophages may be important in cell-cell and cell-matrix adhesive properties of these cells.
层粘连蛋白是一种非胶原细胞外结缔组织糖蛋白，在小鼠腹腔巨噬细胞表面被检测到。通过间接免疫荧光测定，用巯基乙酸盐诱导的多达60%的腹腔巨噬细胞表达细胞表面层粘连蛋白。只有14%的驻留细胞显示可检测到的层粘连蛋白。层粘连蛋白的表达随注射后时间增加。与层粘连蛋白表达同时，巨噬细胞还显示出对来自西非豆的IB4异凝集素的受体。这种凝集素结合甲基-α-D-吡喃半乳糖苷，也可能与层粘连蛋白的碳水化合物部分发生反应。一小部分巨噬细胞同时显示层粘连蛋白和表面纤连蛋白。与驻留细胞和巯基乙酸盐刺激细胞之间层粘连蛋白表达的差异不同，这些细胞群体之间细胞表面纤连蛋白没有差异。由于已发现在其他系统中层粘连蛋白介导细胞附着，这种分子在刺激的巨噬细胞表面的表达可能对这些细胞的细胞间和细胞-基质黏附特性很重要。
