Thompson H L, Burbelo P D, Gabriel G, Yamada Y, Metcalfe D D
Mast Cell Physiology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
J Clin Invest. 1991 Feb;87(2):619-23. doi: 10.1172/JCI115038.
Mast cells are resident in tissues, particularly in association with endothelial and epithelial cell basement membranes, and increase at sites of inflammation, injury, and fibrosis. Although mast cells are known to both release and generate proinflammatory molecules in response to inflammatory stimuli, little is known about their normal biologic function. Here we demonstrate that IL-3-dependent mouse PT18 mast cells, mouse bone marrow-derived mast cells, and rat basophilic leukemia cells express large amounts of mRNA for collagen IV, laminin, and heparan sulfate proteoglycan. Western blot analysis confirmed that mast cells synthesize and secrete significant amounts collagen IV and laminin B1 and B2 chains. These data suggest that mast cells may contribute to normal tissue repair and/or the early overproduction of basement membrane components seen in a variety of fibrotic conditions.
肥大细胞存在于组织中，特别是与内皮细胞和上皮细胞基底膜相关联的部位，并且在炎症、损伤和纤维化部位数量会增加。尽管已知肥大细胞在受到炎症刺激时会释放并产生促炎分子，但其正常生物学功能却知之甚少。在此我们证明，依赖白细胞介素-3的小鼠PT18肥大细胞、小鼠骨髓来源的肥大细胞以及大鼠嗜碱性白血病细胞表达大量的Ⅳ型胶原、层粘连蛋白和硫酸乙酰肝素蛋白聚糖的信使核糖核酸。蛋白质印迹分析证实，肥大细胞合成并分泌大量的Ⅳ型胶原以及层粘连蛋白B1和B2链。这些数据表明，肥大细胞可能有助于正常组织修复和/或在多种纤维化病症中所见的基底膜成分早期过量产生。
