Deleers M, Lebrun P, Malaisse W J
FEBS Lett. 1983 Apr 5;154(1):97-100. doi: 10.1016/0014-5793(83)80882-5.
When rat islets are preincubated with fluorescein diacetate and examined in a spectrofluorometer, the intracellular pH rapidly increases by 0.21 pH units in response to a rise from 1.7-16.8 mM glucose. This coincides with a marked increase in 14CO3H- net uptake by the islets, suggesting that the glucose-induced increase in H+ generation rate is compensated for by stimulation of CO3H-/Cl- exchange.
当用荧光素二乙酸酯对大鼠胰岛进行预孵育并在荧光分光光度计中检测时，随着葡萄糖浓度从1.7 mM升至16.8 mM，细胞内pH值迅速升高0.21个pH单位。这与胰岛对14CO3H-净摄取的显著增加相吻合，表明葡萄糖诱导的H+生成速率增加通过刺激CO3H-/Cl-交换得到补偿。
