Smith M W, Neidhardt F C
J Bacteriol. 1983 Apr;154(1):336-43. doi: 10.1128/jb.154.1.336-343.1983.
The contribution of protein induction and repression to the adaptation of cells to changes in oxygen supply is only poorly understood. We assessed this contribution by measuring the levels of 170 individual polypeptides produced by Escherichia coli K-12 in cells growing aerobically or anaerobically with and without nitrate. Eighteen reached their highest levels during anaerobic growth. These 18 polypeptides include at least 4 glycolytic enzymes and pyruvate formate-lyase (beta-subunit). Most of these proteins were found at significant levels during aerobic growth and appeared to undergo metabolic regulation by stimuli other than anaerobiosis. Anaerobic induction ratios ranged from 1.8- to 11-fold, and nitrate antagonized the anaerobic induction of all of the proteins except one. The time course of synthesis of the proteins after shifts in oxygen supply revealed at least three distinct temporal patterns. These results are discussed in light of known physiological alterations associated with changes in oxygen availability.
蛋白质诱导和阻遏对细胞适应氧气供应变化的贡献目前了解甚少。我们通过测量大肠杆菌K-12在有氧或无氧条件下、有无硝酸盐存在时生长的细胞中产生的170种单个多肽的水平来评估这种贡献。18种多肽在厌氧生长期间达到最高水平。这18种多肽包括至少4种糖酵解酶和丙酮酸甲酸裂解酶（β亚基）。这些蛋白质中的大多数在有氧生长期间以显著水平存在，并且似乎受到除无氧状态以外的其他刺激的代谢调节。厌氧诱导比范围为1.8至11倍，并且硝酸盐拮抗了除一种蛋白质外所有蛋白质的厌氧诱导。氧气供应改变后蛋白质合成的时间进程揭示了至少三种不同的时间模式。根据与氧气可用性变化相关的已知生理改变对这些结果进行了讨论。
