大肠杆菌RNA聚合酶突变体中DNA复制控制的条件性变化
Conditional change of DNA replication control in an RNA polymerase mutant of Escherichia coli.
作者信息
Rasmussen K V, Atlung T, Kerszman G, Hansen G E, Hansen F G
出版信息
J Bacteriol. 1983 Apr;154(1):443-51. doi: 10.1128/jb.154.1.443-451.1983.
Abstract
A temperature-sensitive mutant of Escherichia coli with a temperature-dependent change in the control of initiation of DNA replication was isolated. The phenotype of the mutant was dependent on a mutation in the RNA polymerase gene rpoC. In vitro RNA polymerase activity was temperature sensitive. The mutant grew and synthesized DNA at 30 degrees C as did the wild type. After a shift to 39 degrees C, a temperature still permissive for growth, the mutant increased its origin concentration more than twofold. After a shift from 39 to 30 degrees C, initiation of DNA replication was inhibited until the normal origin concentration was reestablished.
摘要
分离出了一种大肠杆菌的温度敏感突变体，其在DNA复制起始控制方面存在温度依赖性变化。该突变体的表型取决于RNA聚合酶基因rpoC中的一个突变。体外RNA聚合酶活性对温度敏感。该突变体在30℃时的生长和DNA合成情况与野生型相同。在转移至39℃（该温度仍允许生长）后，突变体的起始点浓度增加了两倍多。从39℃转移至30℃后，DNA复制起始受到抑制，直到恢复正常的起始点浓度。
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