Witte P L, Streilein J W
J Immunol. 1983 May;130(5):2282-6.
Monoclonal antibodies were prepared against cell surface antigens present on Syrian hamster lymphocytes and a hamster B cell lymphoma line, GD-36. One of these antibodies, S11, precipitated glycoproteins of 29,000 and 39,000 m.w. These glycoproteins were shown to be identical to or a subset of la-like glycoproteins precipitated by hamster alloantisera; however, molecules identified by S11 differed from the predominant hamster la homologues detected with a cross-reactive monoclonal antibody to murine la.7. The immunofluorescence pattern of both anti-la reagents, S11 and anti-la.7, on hamster lymphoid cells is similar by fluorescence-activated cell sorter analysis. A subpopulation of spleen and lymph node cells stains brightly with these antibodies. By two-color fluorescence, this peripheral lymphocyte subpopulation, identified with monoclonal anti-hamster la, also bears surface immunoglobulin (IgM). These data strongly suggest that hamster resting peripheral B cells, and not T cells, express la antigens and can be identified and isolated differentially by using this marker.
制备了针对叙利亚仓鼠淋巴细胞和仓鼠B细胞淋巴瘤系GD - 36上存在的细胞表面抗原的单克隆抗体。其中一种抗体S11沉淀出分子量为29,000和39,000的糖蛋白。这些糖蛋白被证明与仓鼠同种异体抗血清沉淀的类la糖蛋白相同或为其一个子集；然而，S11鉴定的分子与用针对小鼠la的交叉反应性单克隆抗体检测到的主要仓鼠la同源物不同。通过荧光激活细胞分选分析，抗la试剂S11和抗la.7在仓鼠淋巴细胞上的免疫荧光模式相似。脾脏和淋巴结细胞的一个亚群用这些抗体染色明亮。通过双色荧光，用单克隆抗仓鼠la鉴定的这个外周淋巴细胞亚群也带有表面免疫球蛋白（IgM）。这些数据强烈表明仓鼠静止外周B细胞而非T细胞表达la抗原，并且可以通过使用该标志物进行差异鉴定和分离。
