Sengupta J, Paria B C, Manchanda S K
J Exp Zool. 1983 Jan;225(1):119-22. doi: 10.1002/jez.1402250114.
CI 628 citrate, an estrogen antagonist, was given as a single intraluminal injection (5 micrograms) on day 3 to ovariectomized, progesterone-treated hamsters. This significantly reduced embryo cleavage rate, transformation of morula into blastocyst, and completely inhibited implantation. The effects of the drug could be reversed by estradiol-17B (1 microgram) but not estradiol-17 alpha (1 microgram) injected intraluminally with CI 628 citrate. Our finding suggests a role of estrogen present in hamster preimplantation embryo in the triggering of embryonic differentiation and implantation of the blastocyst.
雌激素拮抗剂CI 628柠檬酸盐在第3天以单次腔内注射（5微克）的方式给予卵巢切除且经孕酮处理的仓鼠。这显著降低了胚胎的分裂率、桑椹胚向囊胚的转化，并完全抑制了着床。该药物的作用可通过腔内注射1微克的雌二醇-17β逆转，但与CI 628柠檬酸盐同时腔内注射1微克的雌二醇-17α则无法逆转其作用。我们的研究结果表明，仓鼠着床前胚胎中存在的雌激素在触发胚胎分化和囊胚着床过程中发挥作用。
