经大鼠盲肠全细胞细菌体外还原后，刚果红和2-偶氮芴转化为诱变剂。

Conversion of Congo red and 2-azoxyfluorene to mutagens following in vitro reduction by whole-cell rat cecal bacteria.

作者信息

Reid T M, Morton K C, Wang C Y, King C M

出版信息

Mutat Res. 1983 Apr;117(1-2):105-12. doi: 10.1016/0165-1218(83)90157-x.

Abstract

Congo red, an azo dye derived from benzidine, and 2-azoxyfluorene, a derivative of 2-aminofluorene, were reduced during overnight incubation with a suspension of rat intestinal bacteria. High performance liquid chromatography and ultraviolet spectral analysis verified the presence of benzidine in extracts of the Congo red incubations and 2-aminofluorene in extracts of the 2-azoxyfluorene incubations. Extracts of the Congo red incubations were mutagenic toward Salmonella typhimurium TA1538 in the presence of a post-mitochondrial activating system, but Congo red was not mutagenic without this reductive pretreatment. Thus, the utility of the Ames test in screening for potential mutagens may be expanded by a reductive pretreatment utilizing cecal bacteria.

摘要

刚果红（一种源自联苯胺的偶氮染料）和2-偶氮芴（2-氨基芴的衍生物）在与大鼠肠道细菌悬浮液过夜孵育期间被还原。高效液相色谱法和紫外光谱分析证实，刚果红孵育提取物中存在联苯胺，2-偶氮芴孵育提取物中存在2-氨基芴。在存在线粒体后激活系统的情况下，刚果红孵育提取物对鼠伤寒沙门氏菌TA1538具有致突变性，但未经这种还原预处理的刚果红没有致突变性。因此，利用盲肠细菌进行还原预处理可能会扩大艾姆斯试验在筛选潜在诱变剂方面的用途。