使用沙门氏菌/阿拉伯糖抗性检测系统对复杂环境混合物进行诱变检测。

Mutagenic detection of complex environmental mixtures using the Salmonella/arabinose-resistant assay system.

作者信息

Whong W Z, Stewart J D, Adamo D C, Ong T

出版信息

Mutat Res. 1983 Apr;120(1):13-9. doi: 10.1016/0165-7992(83)90068-4.

Abstract

The mutagenic sensitivity of SV50, the R-factor plasmid containing tester, of the Salmonella/arabinose-resistant assay system has been evaluated with different environmental complex mixtures, including extracts of airborne and diesel emission particles, oil-shale ash, nitrosated coal dust and water samples. The mutagenicities of all extracts were detectable with this assay. This study indicates that the arabinose-resistant assay with SV50 is useful for the detection of the mutagenic activity of environmental complex mixtures.

摘要

在沙门氏菌/抗阿拉伯糖检测系统中，利用含有测试菌的R因子质粒SV50，针对不同的环境复杂混合物评估了其诱变敏感性，这些混合物包括空气传播颗粒和柴油排放颗粒的提取物、油页岩灰、亚硝化煤粉以及水样。通过该检测方法可检测到所有提取物的诱变性。本研究表明，用SV50进行的抗阿拉伯糖检测对于检测环境复杂混合物的诱变活性是有用的。