Xu J, Whong W Z, Ong T
Mutat Res. 1984 Apr;130(2):79-86. doi: 10.1016/0165-1161(84)90107-9.
Mutagenic sensitivity of the Salmonella/arabinose-resistant (Arar) assay system using the tester strain SV50 was evaluated with 26 compounds both by the preincubation and the standard plate incorporation tests. The mutagenic activity of all 26 compounds was also tested with TA98 and/or TA100 of the Ames Salmonella/microsome assay system. The results indicate that 13 and 10 of 26 compounds were mutagenic and nonmutagenic, respectively, in both assay systems. PR toxin and hydrogen peroxide were mutagenic only in the Arar assay, while 2-nitrofluorene was mutagenic only in the Ames assay. The results also show that the mutagenic response of SV50 to 13 of 15 mutagenic compounds was much higher (2.1-154-fold) if the compounds were tested with the preincubation rather than the plate incorporation test. The mutagenic activity of 4 compounds (diethyl sulfate, niridazole, PR toxin and hydrogen peroxide) in the Arar assay was detected only with the preincubation test. Since the Arar assay using tester strain SV50 has similar mutagenic sensitivity as the Ames assay to chemicals with different modes of action and since it requires only one tester strain, we find this assay system to be useful for screening environmental mutagens. Based on the effectiveness of the preincubation test in this study, it is recommended that the preincubation test instead of the plate incorporation test be used for the Arar assay system with tester strain SV50.
使用测试菌株SV50的沙门氏菌/阿拉伯糖抗性（Arar）检测系统的诱变敏感性，通过预培养试验和标准平板掺入试验，用26种化合物进行了评估。所有26种化合物的诱变活性也用Ames沙门氏菌/微粒体检测系统的TA98和/或TA100进行了测试。结果表明，在这两种检测系统中，26种化合物分别有13种和10种具有诱变活性和无诱变活性。PR毒素和过氧化氢仅在Arar检测中具有诱变活性，而2-硝基芴仅在Ames检测中具有诱变活性。结果还表明，如果用预培养试验而不是平板掺入试验来测试这些化合物，SV50对15种诱变化合物中的13种的诱变反应要高得多（2.1至154倍）。在Arar检测中，4种化合物（硫酸二乙酯、硝唑咪、PR毒素和过氧化氢）的诱变活性仅通过预培养试验检测到。由于使用测试菌株SV50的Arar检测对具有不同作用方式的化学物质具有与Ames检测相似的诱变敏感性，并且由于它只需要一种测试菌株，我们发现这个检测系统对于筛选环境诱变剂很有用。基于本研究中预培养试验的有效性，建议对于使用测试菌株SV50的Arar检测系统，使用预培养试验而非平板掺入试验。
