酵母tRNATyr基因内含子对其tRNA产物的正确修饰至关重要。

The yeast tRNATyr gene intron is essential for correct modification of its tRNA product.

作者信息

Johnson P F, Abelson J

出版信息

Nature. 1983 Apr 21;302(5910):681-7. doi: 10.1038/302681a0.

Abstract

Precise deletion of the intervening sequence of a yeast tRNATyr ochre suppressor gene (SUP6) significantly reduced its suppressor activity relative to that of the unaltered gene. This is probably the result of the absence of the pseudouridine modification, normally present at the middle anticodon position of mature suppressor tRNA, in tRNA synthesized in vivo from the deleted gene.

摘要

精确删除酵母tRNATyr赭石型抑制基因（SUP6）的间隔序列，相对于未改变的基因，其抑制活性显著降低。这可能是由于从缺失基因体内合成的tRNA中，缺少通常存在于成熟抑制性tRNA反密码子中间位置的假尿苷修饰。