酵母赭石抑制基因SUQ5-ol是一种改变的tRNA Ser UCA。
Yeast ochre suppressor SUQ5-ol is an altered tRNA Ser UCA.
作者信息
Waldron C, Cox B S, Wills N, Gesteland R F, Piper P W, Colby D, Guthrie C
出版信息
Nucleic Acids Res. 1981 Jul 10;9(13):3077-88. doi: 10.1093/nar/9.13.3077.
Ochre suppressor tRNA was partially purified from strains of Saccharomyces cerevisiae containing the serine-inserting class III suppressor SUQ5-ol. RNA sequence analysis of this tRNA indicated that the suppressor is derived from a UCA-decoding tRNA Ser by a G leads to U substitution in the middle position of the anticodon. The suppressor further differs from the wild-type UCA-decoding tRNA Ser in that the mutant anticodon lacks the modified uridine found in the wobble position of the wild-type tRNA and contains instead another modification in or near the anticodon.
从含有丝氨酸插入III类抑制子SUQ5 - ol的酿酒酵母菌株中部分纯化了赭石抑制tRNA。对该tRNA的RNA序列分析表明，该抑制子是由UCA解码的tRNA Ser通过反密码子中间位置的G突变为U衍生而来。该抑制子与野生型UCA解码的tRNA Ser的进一步差异在于，突变反密码子缺乏野生型tRNA摆动位置中发现的修饰尿苷，而是在反密码子中或其附近含有另一种修饰。
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