Nelson W J, Lazarides E
Proc Natl Acad Sci U S A. 1983 Jan;80(2):363-7. doi: 10.1073/pnas.80.2.363.
Antibodies raised against electrophoretically purified chicken erythrocyte beta subunit of spectrin, called "beta-spectrin," have been used to demonstrate the presence of an immunoreactive form of this polypeptide in nonerythroid tissues. Immunoautoradiography shows that, in chicken erythrocytes, this antiserum reacts with beta-spectrin (Mr 220,000) and another polypeptide (Mr 230,000) that, by two-dimensional tryptic peptide analysis, shows extensive homology with beta-spectrin but not with the alpha subunit of spectrin, called "alpha-spectrin." Immunoautoradiography and immunoprecipitation of various chicken tissues with this antiserum shows that either one variant or both variants of beta-spectrin are expressed. Indirect immunofluorescence reveals that the antiserum reacts with a plasma membrane-associated component of erythroid and some nonerythroid cells. Particularly strong fluorescence is observed in skeletal and cardiac muscle cells where beta-spectrin appears to form a grid-like network along the inner surface of the sarcolemma. The noncoordinated distribution of alpha- and beta-spectrin variants indicates that their expression may be tailored to the functional requirements of the plasma membrane in different cells.
针对通过电泳纯化的鸡红细胞血影蛋白β亚基（称为“β-血影蛋白”）产生的抗体，已被用于证明该多肽的免疫反应形式在非红细胞组织中的存在。免疫放射自显影显示，在鸡红细胞中，这种抗血清与β-血影蛋白（分子量220,000）和另一种多肽（分子量230,000）发生反应，通过二维胰蛋白酶肽分析，该多肽与β-血影蛋白具有广泛的同源性，但与血影蛋白的α亚基（称为“α-血影蛋白”）没有同源性。用这种抗血清对各种鸡组织进行免疫放射自显影和免疫沉淀表明，β-血影蛋白的一种变体或两种变体均有表达。间接免疫荧光显示，该抗血清与红细胞和一些非红细胞的质膜相关成分发生反应。在骨骼肌和心肌细胞中观察到特别强烈的荧光，其中β-血影蛋白似乎沿着肌膜内表面形成网格状网络。α-和β-血影蛋白变体的不协调分布表明，它们的表达可能是根据不同细胞质膜的功能需求进行调整的。
