Granger B L, Lazarides E
Cell. 1980 Dec;22(3):727-38. doi: 10.1016/0092-8674(80)90549-8.
A 230,000 dalton polypeptide co-purifies through cycles of depolymerization and polymerization with the intermediate filament subunits, desmin and vimentin, from avian smooth muscle. This protein is also present in skeletal muscle and is distinct from myosin and filamin. Double immunofluorescence microscopy of cultured cells, using antisera shown to be specific by immunoautoradiography, has revealed that this protein has the same spatial distribution as desmin and vimentin. During skeletal myogenesis, all three antigens exist initially in multinucleate myotubes as wavy filaments throughout the cytoplasm. Within a week after myoblast fusion, they begin to coalesce at the peripheries of the myofibril Z discs, thereby attaining the distribution observed in mature muscle, a network of interlinked rings within the Z plane. Treatment of cultured myotubes with colcemid causes the filamentous forms of these three proteins to co-aggregate into cytoplasmic bundles, but has little effect on them when they are associated with the Z discs. Extraction of cells with nonionic detergent and high salt leaves cytoskeletons containing desmin, vimentin and the 230,000 dalton polypeptide with immunofluorescent patterns that are indistinguishable from one another. These data suggest that this high molecular weight protein is closely associated with desmin and vimentin filaments in muscle cells; to indicate this, we have named the protein synemin, from the Greek oa uv (with) and v eta mu alpha (filament).
一种230,000道尔顿的多肽与来自禽平滑肌的中间丝亚基结蛋白和波形蛋白一起，通过解聚和聚合循环进行共纯化。这种蛋白质也存在于骨骼肌中，并且与肌球蛋白和细丝蛋白不同。使用免疫放射自显影显示具有特异性的抗血清对培养细胞进行双重免疫荧光显微镜检查，结果表明这种蛋白质与结蛋白和波形蛋白具有相同的空间分布。在骨骼肌生成过程中，所有这三种抗原最初在多核肌管中以波浪状细丝的形式存在于整个细胞质中。成肌细胞融合后一周内，它们开始在肌原纤维Z盘的周边聚集，从而达到在成熟肌肉中观察到的分布，即在Z平面内形成相互连接的环的网络。用秋水仙酰胺处理培养的肌管会导致这三种蛋白质的丝状形式共聚集形成细胞质束，但当它们与Z盘相关联时，对它们几乎没有影响。用非离子洗涤剂和高盐提取细胞后，含有结蛋白、波形蛋白和230,000道尔顿多肽的细胞骨架的免疫荧光模式彼此无法区分。这些数据表明，这种高分子量蛋白质与肌肉细胞中的结蛋白和波形蛋白丝紧密相关；为了表明这一点，我们根据希腊语oa uv（与）和v eta mu alpha（细丝）将这种蛋白质命名为联合细丝蛋白。
