Howden B, Rae D, Jablonski P, Marshall V C, Tange J
Transplantation. 1983 Apr;35(4):311-4. doi: 10.1097/00007890-198304000-00009.
Rat kidneys were flushed with isotonic citrate solution, hypertonic citrate solution, or Collins's C2 solution, and were stored hypothermically for 24 hr before transplantation into another rat of the same inbred colony. The number of animals surviving for one month was greatest with isotonic-citrate-flushed kidneys (82%), and least with Collins's C2 solution (27%). Functional and morphological damage after transplantation was consistently greater in Collins's-flushed grafts, as compared with citrate-flushed grafts. Best results were attained with the isotonic-citrate flushed grafts. Seven days after contralateral nephrectomy all surviving animals had elevated serum creatinine and urea concentrations, along with decreased creatinine clearance, and they had secreted large volumes of dilute urine. Renal function was best in animals with isotonic-citrate-flushed grafts. After one month, significant improvement in urine osmolality, creatinine clearance, and serum creatinine had occurred only in the rats with citrate-flushed grafts. There were no significant differences between the citrate groups. All surviving rats had some residual renal cortical damage, but severe interstitial nephritis (greater than 30%) was much less frequent in the citrate groups.
用等渗柠檬酸盐溶液、高渗柠檬酸盐溶液或柯林斯氏C2溶液冲洗大鼠肾脏，并在低温下保存24小时，然后移植到同一近交系群体的另一只大鼠体内。移植后存活一个月的动物数量，用等渗柠檬酸盐冲洗的肾脏最多（82%），用柯林斯氏C2溶液冲洗的最少（27%）。与柠檬酸盐冲洗的移植物相比，柯林斯氏冲洗的移植物移植后功能和形态学损伤始终更大。等渗柠檬酸盐冲洗的移植物取得了最佳效果。对侧肾切除术后7天，所有存活动物的血清肌酐和尿素浓度升高，同时肌酐清除率降低，并且它们分泌大量稀释尿液。等渗柠檬酸盐冲洗移植物的动物肾功能最佳。一个月后，仅在柠檬酸盐冲洗移植物的大鼠中，尿渗透压、肌酐清除率和血清肌酐有显著改善。柠檬酸盐组之间无显著差异。所有存活大鼠都有一些残余的肾皮质损伤，但柠檬酸盐组中严重间质性肾炎（大于30%）的发生率要低得多。
