Howden B, Jablonski P, Rigol G, Birrell C, Rae D, Marshall V C, Tange J
Transplantation. 1984 Jan;37(1):52-4. doi: 10.1097/00007890-198401000-00015.
This study investigated the possible beneficial effects of reflushing renal grafts with isotonic citrate solution. Rat kidneys were initially flushed with isotonic citrate or with Hartmann's solutions at O C. After 2 hr, half the kidneys of each group were reflushed with isotonic citrate; 22 hr later, all kidneys were transplanted into rats of the same inbred strain. All animals receiving kidneys flushed with Hartmann's solution died, whereas reflushing such kidneys with isotonic citrate significantly ameliorated the deleterious effects of Hartmann's solution. All animals receiving citrate-flushed kidneys survived with relatively good renal function and morphology. However, reflushing itself is not a beneficial procedure and is only of value where an ineffective preserving solution has been used to flush the kidneys initially. There is evidence that some of the adverse effects of flushing develop in the renal medulla.
本研究调查了用等渗柠檬酸盐溶液再次冲洗肾移植物可能产生的有益效果。大鼠肾脏最初在0℃时用等渗柠檬酸盐溶液或哈特曼氏溶液冲洗。2小时后，每组一半的肾脏用等渗柠檬酸盐溶液再次冲洗；22小时后，将所有肾脏移植到同一近交系大鼠体内。所有接受用哈特曼氏溶液冲洗过的肾脏的动物均死亡，而用等渗柠檬酸盐溶液对这类肾脏进行再次冲洗可显著改善哈特曼氏溶液的有害影响。所有接受用柠檬酸盐溶液冲洗过的肾脏的动物均存活，且肾功能和形态相对良好。然而，再次冲洗本身并非有益的操作，仅在最初使用无效的保存液冲洗肾脏时才有价值。有证据表明，冲洗的一些不良影响发生在肾髓质。
