Visser R G, Hellingwerf K J, Konings W N
J Bioenerg Biomembr. 1984 Aug;16(4):295-307. doi: 10.1007/BF00744282.
The protein composition of the cytoplasmic membranes of Escherichia coli, grown aerobically and anaerobically on a glucose minimal medium at pH 7.0, were analyzed by crossed immunoelectrophoresis. Qualitative differences are limited to only two proteins: nitrate reductase (E.C. 1.7.99.4) is absent under aerobic growth conditions, whereas an unidentified protein, with a molecular weight of 81,500 and located at the inner side of the cytoplasmic membrane, is synthesized only in the presence of oxygen. Quantitative differences are observed for many proteins: the ratio of the amount of a specific protein present in cells grown anaerobically and aerobically was, for four proteins, between 0.3 and 1; for 25 proteins, between 1 and 3; and for five proteins, larger than 5.
采用交叉免疫电泳法分析了在pH 7.0的葡萄糖基本培养基上进行好氧和厌氧培养的大肠杆菌细胞质膜的蛋白质组成。定性差异仅局限于两种蛋白质：在好氧生长条件下不存在硝酸还原酶（E.C. 1.7.99.4），而一种分子量为81,500且位于细胞质膜内侧的未鉴定蛋白质仅在有氧条件下合成。观察到许多蛋白质存在定量差异：四种蛋白质在厌氧生长和好氧生长的细胞中存在的特定蛋白质数量之比在0.3至1之间；25种蛋白质在1至3之间；还有五种蛋白质大于5。
