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大肠杆菌细胞色素bo3中电子转移的热力学

Thermodynamics of electron transfer in Escherichia coli cytochrome bo3.

作者信息

Schultz B E, Chan S I

机构信息

Arthur Amos Noyes Laboratory of Chemical Physics, California Institute of Technology, Pasadena, CA 91125, USA.

出版信息

Proc Natl Acad Sci U S A. 1998 Sep 29;95(20):11643-8. doi: 10.1073/pnas.95.20.11643.

DOI:10.1073/pnas.95.20.11643
PMID:9751719
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC21694/
Abstract

The proton translocation mechanism of the Escherichia coli cytochrome bo3 complex is intimately tied to the electron transfers within the enzyme. Herein we evaluate two models of proton translocation in this enzyme, a cytochrome c oxidase-type ion-pump and a Q-cycle mechanism, on the basis of the thermodynamics of electron transfer. We conclude that from a thermodynamic standpoint, a Q-cycle is the more favorable mechanism for proton translocation and is likely occurring in the enzyme.

摘要

大肠杆菌细胞色素bo3复合物的质子转运机制与酶内的电子传递密切相关。在此，我们基于电子传递的热力学原理，评估了该酶中质子转运的两种模型，即细胞色素c氧化酶型离子泵和Q循环机制。我们得出结论，从热力学角度来看，Q循环是更有利于质子转运的机制，并且很可能在该酶中发生。

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本文引用的文献

1
Reaction of Escherichia coli cytochrome bo3 with substoichiometric ubiquinol-2: a freeze-quench electron paramagnetic resonance investigation.大肠杆菌细胞色素bo3与亚化学计量泛醇-2的反应：冷冻淬灭电子顺磁共振研究
Biochemistry. 1998 Mar 24;37(12):4160-8. doi: 10.1021/bi971714y.
2
Glutamic acid 286 in subunit I of cytochrome bo3 is involved in proton translocation.细胞色素bo3亚基I中的谷氨酸286参与质子转运。
Proc Natl Acad Sci U S A. 1997 Sep 16;94(19):10128-31. doi: 10.1073/pnas.94.19.10128.
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Uncompetitive substrate inhibition and noncompetitive inhibition by 5-n-undecyl-6-hydroxy-4,7-dioxobenzothiazole (UHDBT) and 2-n-nonyl-4-hydroxyquinoline-N-oxide (NQNO) is observed for the cytochrome bo3 complex: implications for a Q(H2)-loop proton translocation mechanism.对于细胞色素bo3复合物，观察到5-正十一烷基-6-羟基-4,7-二氧代苯并噻唑（UHDBT）和2-正壬基-4-羟基喹啉-N-氧化物（NQNO）的非竞争性底物抑制和非竞争性抑制：对Q(H2)-环质子转运机制的影响。
Biochemistry. 1997 Jan 28;36(4):894-902. doi: 10.1021/bi961723r.
4
The "ferrous-oxy" intermediate in the reaction of dioxygen with fully reduced cytochromes aa3 and bo3.氧气与完全还原的细胞色素aa3和bo3反应中的“亚铁-氧”中间体。
Biochemistry. 1996 Dec 17;35(50):16241-6. doi: 10.1021/bi961433a.
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Cytochrome c oxidase: chemistry of a molecular machine.细胞色素c氧化酶：分子机器的化学原理
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6
The whole structure of the 13-subunit oxidized cytochrome c oxidase at 2.8 A.13亚基氧化型细胞色素c氧化酶在2.8埃分辨率下的整体结构。
Science. 1996 May 24;272(5265):1136-44. doi: 10.1126/science.272.5265.1136.
7
Flow-flash study of the reaction between cytochrome bo and oxygen.
Biochemistry. 1993 May 25;32(20):5442-7. doi: 10.1021/bi00071a021.
8
Substitution of asparagine for aspartate-135 in subunit I of the cytochrome bo ubiquinol oxidase of Escherichia coli eliminates proton-pumping activity.将大肠杆菌细胞色素bo泛醇氧化酶亚基I中的天冬氨酸-135替换成天冬酰胺会消除质子泵活性。
Biochemistry. 1993 Oct 12;32(40):10923-8. doi: 10.1021/bi00091a048.
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Comparison of ubiquinol and cytochrome c terminal oxidases. An alternative view.泛醇与细胞色素c末端氧化酶的比较。另一种观点。
FEBS Lett. 1993 Jul 26;327(2):131-6. doi: 10.1016/0014-5793(93)80156-o.
10
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FEBS Lett. 1993 Jul 5;325(3):299-302. doi: 10.1016/0014-5793(93)81093-f.