大肠杆菌细胞色素bo3中电子转移的热力学
Thermodynamics of electron transfer in Escherichia coli cytochrome bo3.
作者信息
Schultz B E, Chan S I
机构信息
Arthur Amos Noyes Laboratory of Chemical Physics, California Institute of Technology, Pasadena, CA 91125, USA.
出版信息
Proc Natl Acad Sci U S A. 1998 Sep 29;95(20):11643-8. doi: 10.1073/pnas.95.20.11643.
Abstract
The proton translocation mechanism of the Escherichia coli cytochrome bo3 complex is intimately tied to the electron transfers within the enzyme. Herein we evaluate two models of proton translocation in this enzyme, a cytochrome c oxidase-type ion-pump and a Q-cycle mechanism, on the basis of the thermodynamics of electron transfer. We conclude that from a thermodynamic standpoint, a Q-cycle is the more favorable mechanism for proton translocation and is likely occurring in the enzyme.
摘要
大肠杆菌细胞色素bo3复合物的质子转运机制与酶内的电子传递密切相关。在此，我们基于电子传递的热力学原理，评估了该酶中质子转运的两种模型，即细胞色素c氧化酶型离子泵和Q循环机制。我们得出结论，从热力学角度来看，Q循环是更有利于质子转运的机制，并且很可能在该酶中发生。
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本文引用的文献
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2
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3
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