Tsubakio T, Kurata Y, Yonezawa T, Kitani T
Acta Haematol. 1981;66(4):251-6. doi: 10.1159/000207130.
In order to measure platelet-associated IgG (PAIgG), we devised a solid-phase enzyme immunoassay employing a competitive binding of peroxidase-conjugated anti-IgG antiserum between platelets and polystyrene tubes coated with IgG. The amounts of peroxidase bound to the tubes were measured in a spectrophotometer by an enzymatic reaction. This method is highly sensitive, reproducible and can be carried out more simply. the PAIgG values of normal controls averaged 21.6 +/- 6.6 (SD) ng/10(7) platelets. 27 (93%) of 29 patients with idiopathic thrombocytopenic purpura (ITP), who had a platelet count of less than 15 X 10(4)/microliter, had PAIgG values greater than those of controls by 2 SD and averaged 205.5 +/- 323 ng. There was a significant inverse correlation between platelet count and PAIgG value of ITP patients. the PAIgG values of patients with aplastic anemia were within normal range.
为了检测血小板相关IgG（PAIgG），我们设计了一种固相酶免疫测定法，该方法利用过氧化物酶结合的抗IgG抗血清在血小板与包被有IgG的聚苯乙烯管之间的竞争性结合。通过酶促反应在分光光度计中测量与管结合的过氧化物酶的量。该方法高度灵敏、可重复且操作更简便。正常对照的PAIgG值平均为21.6±6.6（标准差）ng/10⁷血小板。29例特发性血小板减少性紫癜（ITP）患者中，血小板计数低于15×10⁴/微升的27例（93%），其PAIgG值比对照组高2个标准差，平均为205.5±323 ng。ITP患者的血小板计数与PAIgG值之间存在显著的负相关。再生障碍性贫血患者的PAIgG值在正常范围内。
