Hahn B, Manzari V, Colombini S, Franchini G, Gallo R C, Wong-Staal F
Nature. 1983;303(5914):253-6. doi: 10.1038/303253a0.
Human T-cell leukaemia-lymphoma virus (HTLV) was first isolated in the United States from a patient with an aggressive form of cutaneous T-cell lymphoma (CTCL) and was later found associated with clusters of adult T-cell leukaemia-lymphomas (ATL) in various parts of the world, including Japan and the Caribbean. Leukaemic cells of the HTLV-positive patients seem to be clonal expansions of single infected cells since the provirus(es) are found at the same sites in a given patient. In avian leukosis virus-induced B-cell lymphomas, the provirus very frequently integrates at several discrete sites in a common domain near the cellular gene, c-myc, that it activates and it has been speculated that the same would hold true for other chronic leukaemia viruses. We report here that cultured cells from two US patients with CTCL and fresh leukaemia cells of a Japanese patient with ATL contained an HTLV provirus integrated at the same site. In addition, a cord blood T-lymphocyte cell line established by co-cultivation with one of the two HTLV-positive CTCL cell lines also contained HTLV provirus contiguous with the same flanking cellular sequences. Ten other HTLV-positive cell samples did not show integration of HTLV at this site, suggesting that there is more than one discrete site of HTLV integration in tumour cells.
人类T细胞白血病-淋巴瘤病毒（HTLV）最初是在美国从一名患有侵袭性皮肤T细胞淋巴瘤（CTCL）的患者体内分离出来的，后来在世界不同地区，包括日本和加勒比地区，发现它与成人T细胞白血病-淋巴瘤（ATL）集群有关。HTLV阳性患者的白血病细胞似乎是单个感染细胞的克隆扩增，因为在给定患者的相同位点发现了前病毒。在禽白血病病毒诱导的B细胞淋巴瘤中，前病毒非常频繁地整合到细胞基因c-myc附近一个共同区域的几个离散位点，它激活了该基因，并且有人推测其他慢性白血病病毒也会如此。我们在此报告，两名美国CTCL患者的培养细胞以及一名日本ATL患者的新鲜白血病细胞中，HTLV前病毒整合在相同位点。此外，与两个HTLV阳性CTCL细胞系之一共同培养建立的脐带血T淋巴细胞系也含有与相同侧翼细胞序列相邻的HTLV前病毒。其他十个HTLV阳性细胞样本在该位点未显示HTLV整合，这表明肿瘤细胞中存在不止一个离散的HTLV整合位点。
