Markham P D, Salahuddin S Z, Kalyanaraman V S, Popovic M, Sarin P, Gallo R C
Int J Cancer. 1983 Apr 15;31(4):413-20. doi: 10.1002/ijc.2910310404.
Human T-cell leukemia-lymphoma virus (HTLV) was first isolated from sporadic patients with adult T-cell malignancies in the United States and subsequently from T-lymphocytes established in culture from additional T-cell leukemia-lymphoma patients living in different geographical areas of the world. Co-cultivation of normal umbilical cord blood with lethally irradiated, HTLV-positive lymphocytes established in culture from many of these patients resulted in the productive infection of the cord blood T-lymphocytes which grew in suspension culture in the absence of exogenous TCGF. These transformed cord blood cells have morphological and cytochemical properties similar to HTLV-positive fresh and cultured tumor T-cells and are distinguishable from virus donor cells by HLA haplotype and chromosomal markers. These cells express HTLV proteins, release type-C virus particles and contain surface receptors for TCGF. These results demonstrate that HTLV isolated from T-cell leukemic donors from different parts of the world can productively infect and transform fresh human cord blood T-lymphocytes, and that the transformed cells share many similarities with fresh or cultured leukemic cells.
人类T细胞白血病-淋巴瘤病毒（HTLV）最初是从美国散发性成人T细胞恶性肿瘤患者中分离出来的，随后又从世界不同地理区域的其他T细胞白血病-淋巴瘤患者的培养淋巴细胞中分离得到。将正常脐带血与来自许多此类患者的经致死剂量照射的HTLV阳性培养淋巴细胞共同培养，导致脐带血T淋巴细胞发生有效感染，这些细胞在无外源性TCGF的悬浮培养中生长。这些转化的脐带血细胞具有与HTLV阳性新鲜及培养肿瘤T细胞相似的形态学和细胞化学特性，并且通过HLA单倍型和染色体标记可与病毒供体细胞区分开来。这些细胞表达HTLV蛋白，释放C型病毒颗粒，并含有TCGF的表面受体。这些结果表明，从世界不同地区的T细胞白血病供体中分离出的HTLV可有效感染并转化新鲜人脐带血T淋巴细胞，且转化细胞与新鲜或培养的白血病细胞有许多相似之处。
