Osborne N N
Neurosci Lett. 1983 Jan 31;35(1):15-8. doi: 10.1016/0304-3940(83)90519-0.
Using high performance liquid chromatography (HPLC) and electrochemical detection, serotonin and its precursor 5-hydroxytryptophan (5-HTP) were identified in crude perchloric acid extracts from bovine cornea. In addition, dopamine, noradrenaline and adrenaline were identified. Through the use of immunohistochemical procedures it was established that the serotonin is localized in nerve fibers in the cornea and they were always found subepithelially or in the stroma. The occurrence of serotonergic fibres in the cornea suggests that the amine has a specific functional role in this tissue.
采用高效液相色谱（HPLC）和电化学检测法，在牛角膜的高氯酸粗提物中鉴定出了血清素及其前体5-羟色氨酸（5-HTP）。此外，还鉴定出了多巴胺、去甲肾上腺素和肾上腺素。通过免疫组织化学方法确定，血清素定位于角膜的神经纤维中，且总是在上皮细胞下或基质中发现。角膜中血清素能纤维的存在表明该胺在该组织中具有特定的功能作用。
