Nesselhut T, Osborne N N
Neurosci Lett. 1982 Jan 22;28(1):41-5. doi: 10.1016/0304-3940(82)90205-1.
Using high performance liquid chromatography (HPLC) and electrochemical detection, a major substance corresponding to noradrenaline was identified in crude perchloric acid extracts from bovine retina. Prior purification of the extract with aluminium oxide followed by HPLC confirmed this observation. By altering the oxidation potential of the detector, noradrenaline was confirmed by a further criterion. Bovine retina was also shown to metabolize [3H]dopamine to form a substance which was identified as noradrenaline by the same criteria used to establish its presence. The noradrenaline content was found to be 62 +/- 4 ng/g fresh weight of tissue. Its level is therefore of the same order as the dopamine content (86 +/- 3 ng/g fresh weight).
采用高效液相色谱（HPLC）和电化学检测法，在牛视网膜的高氯酸粗提物中鉴定出一种与去甲肾上腺素相对应的主要物质。先用氧化铝对提取物进行预纯化，再进行HPLC分析，证实了这一观察结果。通过改变检测器的氧化电位，依据另一标准证实了去甲肾上腺素的存在。还发现牛视网膜可将[3H]多巴胺代谢形成一种物质，采用与确定其存在相同的标准将该物质鉴定为去甲肾上腺素。结果发现去甲肾上腺素含量为62±4 ng/g组织鲜重。因此，其水平与多巴胺含量（86±3 ng/g鲜重）处于同一数量级。
