Babaev V R, Shchetnikova L A
Arkh Patol. 1983;45(1):76-8.
Using an indirect immunoperoxidase technique, the methods of fixation and treatment of paraffin sections were explored for subsequent identification of myosin of smooth and skeletal muscle cells, actin, filamin, and immunoglobulins. Bouin's fluid and ethanol were found to be the most adequate fixators for immunomorphological analysis. Pronase treatment of paraffin sections increased the intensity of specific reactions considerably. Incubation of paraffin sections with normal goat serum and hydrogen peroxide decreased nonspecific staining of the sections.
采用间接免疫过氧化物酶技术，探索了石蜡切片的固定和处理方法，以便后续鉴定平滑肌细胞和骨骼肌细胞的肌球蛋白、肌动蛋白、细丝蛋白及免疫球蛋白。发现Bouin氏液和乙醇是免疫形态学分析中最合适的固定剂。对石蜡切片进行链霉蛋白酶处理可显著增强特异性反应的强度。用正常山羊血清和过氧化氢孵育石蜡切片可减少切片的非特异性染色。
