用酶联免疫吸附测定法检测梅毒患者脑脊液中的免疫球蛋白M
Detection of immunoglobulin M in cerebrospinal fluid from syphilis patients by enzyme-linked immunosorbent assay.
作者信息
Lee J B, Farshy C E, Hunter E F, Hambie E A, Wobig G H, Larsen S A
出版信息
J Clin Microbiol. 1986 Nov;24(5):736-40. doi: 10.1128/jcm.24.5.736-740.1986.
Cerebrospinal fluid (CSF) samples were evaluated in an immunoglobulin M enzyme-linked immunosorbent assay (IgM ELISA) for syphilis with sonic extracts of Treponema pallidum coated on polystyrene plates. The ELISA procedure was reproducible, and T. pallidum antigens were stable., A total of 15 CSF samples from patients with neurosyphilis, 18 CSF samples from patients with syphilis, 12 CSF samples from patients treated for syphilis, and 494 CSF samples from patients with neurologic or other systemic diseases were tested. The IgM ELISA gave reactive results in all of six symptomatic and congenital neurosyphilitic patients and none of nine asymptomatic neurosyphilitic patients. Of 524 CSF samples from nonneurosyphilitic individuals, 513 were nonreactive, resulting in 98% test specificity. The IgM ELISA in CSF should prove to be useful for confirmation of symptomatic neurosyphilis.
在一项免疫球蛋白M酶联免疫吸附测定（IgM ELISA）中，使用包被在聚苯乙烯板上的梅毒螺旋体超声提取物对脑脊液（CSF）样本进行梅毒检测。ELISA程序具有可重复性，梅毒螺旋体抗原稳定。共检测了15份来自神经梅毒患者的脑脊液样本、18份来自梅毒患者的脑脊液样本、12份来自接受梅毒治疗患者的脑脊液样本以及494份来自患有神经系统疾病或其他全身性疾病患者的脑脊液样本。IgM ELISA在所有6例有症状的先天性神经梅毒患者中均给出阳性结果，而在9例无症状神经梅毒患者中均为阴性。在524份来自非神经梅毒个体的脑脊液样本中，513份为阴性，检测特异性为98%。脑脊液中的IgM ELISA应被证明对确诊有症状的神经梅毒有用。
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