Tetaz T J, Luke R K
J Bacteriol. 1983 Jun;154(3):1263-8. doi: 10.1128/jb.154.3.1263-1268.1983.
The copper resistance of a strain of Escherichia coli isolated from the effluent of a piggery where pigs were fed a diet supplemented with copper sulfate was controlled by a conjugative 78-megadalton plasmid designated pRJ1004. Plasmid pRJ1004 exhibited surface exclusion and incompatibility with standard plasmids belonging to incompatibility groups I1 and K. Sensitive strains of E. coli K-12 were unable to form colonies on nutrient agar containing more than 4 mM copper, whereas transconjugants which harbored pRJ1004 were able to form colonies on medium containing up to 20 mM copper.
从一个用添加了硫酸铜的饲料喂养猪的养猪场废水中分离出的一株大肠杆菌的铜抗性,由一个名为pRJ1004的78兆道尔顿接合质粒控制。质粒pRJ1004表现出表面排斥作用,并且与属于不相容群I1和K的标准质粒不相容。大肠杆菌K-12的敏感菌株在含有超过4 mM铜的营养琼脂上无法形成菌落,而携带pRJ1004的转接合子能够在含有高达20 mM铜的培养基上形成菌落。