Davey R B, Pittard J
Aust J Exp Biol Med Sci. 1980 Aug;58(4):331-8. doi: 10.1038/icb.1980.32.
Three plasmids which each determined the same extensive antibiotic resistance phenotype, including resistance to gentamicin, and which had been recovered from organisms involved in an outbreak of gentamicin-resistant infections in Melbourne hospitals in 1975--1976, were analysed by fingerprinting with the restriction endonucleases Eco RI, Hind III and Pst I, In each case the fingerprints for all three plasmids were identical, despite the fact that each plasmid originated in a different host species at different hospitals. This observation confirms our earlier hypothesis (Davey and Pittard, 1977) that the plasmids mediating gentamicin resistance in the outbreak were the closely related descendants of one ancestral (IncL) plasmid.
对从1975年至1976年墨尔本医院耐庆大霉素感染暴发中涉及的生物体中回收的三种质粒进行了分析，这三种质粒各自决定相同的广泛抗生素耐药表型，包括对庆大霉素的耐药性。使用限制性内切酶Eco RI、Hind III和Pst I进行指纹分析。在每种情况下，尽管每种质粒起源于不同医院的不同宿主物种，但所有三种质粒的指纹都是相同的。这一观察结果证实了我们早期的假设（戴维和皮塔德，1977年），即在此次暴发中介导庆大霉素耐药性的质粒是一个祖先（IncL）质粒的密切相关后代。
