Chung C P, Nisengard R, Slots J, Ciancio S
J Periodontol. 1983 Apr;54(4):236-46. doi: 10.1902/jop.1983.54.4.236.
Serum antibodies to indigenous bacteria in ten beagle dogs were examined over a 7-month period during the development of ligature-induced periodontitis. Gram-negative strains comprised approximately 75% of the cultivable periodontitis microflora with a predominance of black-pigmented Bacteroides species. A total of 44 bacterial strains representing the predominant cultivable subgingival beagle dog microflora was selected for antibody determination. The IgG and, in some cases, IgM serum antibody titers to these organisms were determined by indirect immunofluorescence. The antibody titers to most test strains remained unchanged during the experimental period. Gram-negative bacteria generally exhibited lower titers than the Gram-positive bacteria. Especially low titers were found for the black-pigmented Bacteroides. Four dogs that developed the most severe periodontitis showed about 2-fold higher IgG titers to some Gram-negative anaerobic rods in the pre-ligation period than dogs that developed a more moderate periodontitis. These data suggested a possible diagnostic value of such antibody determinations. However, the overall finding of the present study was that serum antibody titers to key periodontopathic organisms remained low throughout the experiment. This result may suggest that the rapid periodontal destruction in ligature-induced periodontitis is due in part to an inadequate antibody response against the infecting microorganisms and their pathogenic products.
在结扎诱导的牙周炎发展过程中，对10只比格犬的血清中针对本土细菌的抗体进行了为期7个月的检测。革兰氏阴性菌株约占可培养的牙周炎微生物群的75%，其中以产黑色素类杆菌为主。共选择了44株代表比格犬龈下主要可培养微生物群的菌株进行抗体测定。通过间接免疫荧光法测定血清中针对这些微生物的IgG抗体效价，在某些情况下还测定了IgM抗体效价。在实验期间，大多数测试菌株的抗体效价保持不变。革兰氏阴性菌的效价通常低于革兰氏阳性菌。产黑色素类杆菌的效价尤其低。与患中度牙周炎的犬相比，4只患最严重牙周炎的犬在结扎前对某些革兰氏阴性厌氧杆菌的IgG效价高出约2倍。这些数据表明这种抗体测定可能具有诊断价值。然而，本研究的总体结果是，在整个实验过程中，针对关键牙周病原菌的血清抗体效价一直很低。这一结果可能表明，结扎诱导的牙周炎中快速的牙周破坏部分归因于针对感染微生物及其致病产物的抗体反应不足。
