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链霉菌噬菌体SH10的DNA：大肠杆菌RNA聚合酶的结合位点及变性图谱。

DNA of the Streptomyces phage SH10: binding sites for Escherichia coli RNA polymerase and denaturation map.

作者信息

Klaus S, Vogel F, Gautschi J, Stålhammar-Carlemalm M, Meyer J

出版信息

Mol Gen Genet. 1983;189(1):21-6. doi: 10.1007/BF00326050.

DOI:10.1007/BF00326050
PMID:6343784
Abstract

Escherichia coli RNA polymerase bound to Streptomyces phage SH10 DNA was visualized by electron microscopy. Six specific binding sites were observed at map units 53, 85, 93, 97, 98, and 99 on the physical map of the 48 kb long genome. Electron microscopy of partially denatured SH10 DNA revealed a characteristic melting pattern of A + T-rich regions around map units 1, 3, 48, 52, and 99. A comparison of the denaturation map with the RNA polymerase binding sites indicates that three binding sites are located in the most A + T-rich regions, two in other early melting regions and one in a segment of higher DNA helix stability.

摘要

通过电子显微镜观察到与链霉菌噬菌体SH10 DNA结合的大肠杆菌RNA聚合酶。在48 kb长基因组的物理图谱上，于图谱单位53、85、93、97、98和99处观察到六个特异性结合位点。部分变性的SH10 DNA的电子显微镜观察揭示了图谱单位1、3、48、52和99周围富含A+T区域的特征性解链模式。变性图谱与RNA聚合酶结合位点的比较表明，三个结合位点位于A+T含量最高的区域，两个位于其他早期解链区域，一个位于DNA螺旋稳定性较高的片段中。

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Mol Gen Genet. 1983;189(1):21-6. doi: 10.1007/BF00326050.
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本文引用的文献

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A comparison of the phage T4 gene 32 protein and Escherichia coli RNA polymerase binding sites on hamster papovavirus DNA.
Biochim Biophys Acta. 1982 Jan 26;696(1):102-6. doi: 10.1016/0167-4781(82)90015-x.
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