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噬菌体φ29 DNA中富含A-T区域与枯草芽孢杆菌RNA聚合酶结合位点的比较。

Comparison of the A-T rich regions and the Bacillus subtilis RNA polymerase binding sites in phage phi 29 DNA.

作者信息

Sogo J M, Rodeño P, Koller T, Viñuela E, Salas M

出版信息

Nucleic Acids Res. 1979 Sep 11;7(1):107-20. doi: 10.1093/nar/7.1.107.

DOI:10.1093/nar/7.1.107
PMID:114982
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327999/
Abstract

By using a modification of the BAC spreading method for mounting the DNA for electron microscopy, partial denaturation maps of protein-free phi 29 DNA and of phi 29 DNA containing protein p3 were obtained. In phi 29 p3-DNA1 the protein does not seem to influence the melting of the ends of the molecules. The comparison of the partial denaturation map and the B. subtilis RNA polymerase binding sites indicates that five of the seven early promoters (A1, A2, A3, B2 and C2) are located in A-T rich DNA regions whereas the other two early promoters (B1 and C1) are located in less A-T rich sites.

摘要

通过对用于电子显微镜观察的DNA进行安装的BAC铺展方法进行改进，获得了无蛋白质的phi 29 DNA和含有蛋白质p3的phi 29 DNA的部分变性图谱。在phi 29 p3-DNA1中，该蛋白质似乎不影响分子末端的解链。部分变性图谱与枯草芽孢杆菌RNA聚合酶结合位点的比较表明，七个早期启动子中的五个（A1、A2、A3、B2和C2）位于富含A-T的DNA区域，而另外两个早期启动子（B1和C1）位于富含A-T较少的位点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df64/327999/716f805e1bc4/nar00454-0119-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df64/327999/2608e76c702e/nar00454-0114-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df64/327999/d5b301e4d95e/nar00454-0116-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df64/327999/716f805e1bc4/nar00454-0119-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df64/327999/2608e76c702e/nar00454-0114-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df64/327999/d5b301e4d95e/nar00454-0116-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df64/327999/716f805e1bc4/nar00454-0119-a.jpg

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Mol Gen Genet. 1983;189(1):21-6. doi: 10.1007/BF00326050.
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