Chamla Y, Ruffié M
Hum Genet. 1976 Oct 28;34(2):213-6. doi: 10.1007/BF00278891.
A modified thermal denaturation of human chromosomes in Hanks' solution at 91 degrees C produces highly contrasted C and T bands. This improved double-banding system is a good tool in the analysis of ring chromosomes, for which R banding is often inadequate, as it is exemplified.