Arenzana-Seisdedos F, Virelizier J L
Eur J Immunol. 1983 Jun;13(6):437-40. doi: 10.1002/eji.1830130602.
Human adherent monocytes were incubated with interferon (IFN) preparations, then washed and stimulated with endotoxins. The interleukin (IL1) activity in the supernatants of IFN-treated monocyte cultures was found to be increased as compared to control (not pretreated) cultures. This phenomenon was observed whether alpha or beta IFN was used, and was completely abolished by antiserum to the relevant IFN. IL1 secretion of IFN-treated monocyte cultures could be triggered by suboptimal dosage of endotoxins, unable to induce any IL1 activity in control medium-treated cultures. IFN was highly efficient in this system, since very low concentrations (of the order of 2 units) were effective. Since IFN alone, without endotoxin stimulation, was unable to induce IL1 secretion, the results indicate that IFN is able to activate monocytes, by inducing a potential secretory capable rather than an ongoing IL1 secretion function. The ability of IFN to enhance the IL1 secretory potential of human monocytes may be relevant to the known immunoenhancing effects of IFN preparations and to the pyrogenic effects of IFN administration in patients.
将人贴壁单核细胞与干扰素（IFN）制剂一起孵育，然后洗涤并用内毒素刺激。与对照（未预处理）培养物相比，发现IFN处理的单核细胞培养物上清液中的白细胞介素（IL1）活性增加。无论使用α-IFN还是β-IFN，均观察到这种现象，并且相关IFN的抗血清可完全消除该现象。IFN处理的单核细胞培养物的IL1分泌可由次优剂量的内毒素触发，而次优剂量的内毒素在对照培养基处理的培养物中不能诱导任何IL1活性。IFN在该系统中效率很高，因为非常低的浓度（约2个单位）就有效。由于单独的IFN在没有内毒素刺激的情况下不能诱导IL1分泌，结果表明IFN能够通过诱导潜在的分泌能力而不是持续的IL1分泌功能来激活单核细胞。IFN增强人单核细胞IL1分泌潜力的能力可能与IFN制剂已知的免疫增强作用以及IFN给药对患者的致热作用有关。
