Liu J H, Durfee R, Muse K, Yen S S
Fertil Steril. 1983 Jul;40(1):18-22.
Pharmacologic doses of gonadotropin-releasing hormone (GnRH) pulses (10 micrograms) were used for the induction of multiple follicular development in six normal cycling women. GnRH administration via an autoinfusion pump (intravenously at 60- to 120-minute intervals) was begun on day 5 after the onset of menses. During these stimulated cycles, two to five follicles of preovulatory size were detected by sonography in all subjects. In two subjects, follicle aspiration was performed with successful oocyte recovery. Assessment of the follicular fluid steroid concentrations from these follicles suggests that two of three (subject 5) and four of five (subject 6) follicles were mature. Gonadotropin levels of treated cycles were significantly higher than in normal cycles. Both follicular and luteal phases were of normal duration but were associated with significantly elevated estradiol and progesterone levels, respectively. Timely midcycle gonadotropin surges occurred by days 12 to 14 in all cases. Thus, ovarian steroid feedback control of gonadotropin secretion can be overridden by larger pulses of GnRH, resulting in the development of multiple follicles. This technique may prove useful for in vitro fertilization.
采用药理剂量的促性腺激素释放激素（GnRH）脉冲（10微克）诱导6名月经周期正常的女性多个卵泡发育。在月经开始后的第5天，通过自动输注泵（每隔60至120分钟静脉注射一次）给予GnRH。在这些刺激周期中，通过超声检查在所有受试者中均检测到两至五个排卵前大小的卵泡。在两名受试者中，进行了卵泡抽吸并成功回收了卵母细胞。对这些卵泡的卵泡液类固醇浓度评估表明，三名受试者中的两名（受试者5）和五名受试者中的四名（受试者6）卵泡成熟。治疗周期中的促性腺激素水平显著高于正常周期。卵泡期和黄体期持续时间均正常，但分别与雌二醇和孕酮水平显著升高有关。在所有病例中，在第12至14天出现了适时的周期中期促性腺激素高峰。因此，更大脉冲的GnRH可以克服卵巢类固醇对促性腺激素分泌的反馈控制，从而导致多个卵泡发育。该技术可能对体外受精有用。
