Melville-Smith M E, Seagroatt V A, Watkins J T
J Biol Stand. 1983 Apr;11(2):137-44. doi: 10.1016/s0092-1157(83)80038-9.
An enzyme-linked immunosorbent assay (ELISA) has been developed for the measurement of tetanus antitoxin in human sera as an alternative to the toxin neutralization test in mice, the currently accepted method of assay. The ELISA was found to be simple and quick to perform and required only small amounts of materials. In addition, the assay was found to give reproducible estimates of antitoxin levels and to measure antitoxin at levels as low as 0.01 IU per ml, a sensitivity similar to that of the neutralization test. Furthermore, a comparison of the results of the ELISA and the neutralization test involving 80 human sera, including sera with both high and low antitoxin levels, showed close agreement in antitoxin levels obtained by the two methods. It was concluded that ELISA was an acceptable alternative to the toxin neutralization test in mice for the measurement of tetanus antitoxin levels in human sera.
已开发出一种酶联免疫吸附测定法（ELISA）用于检测人血清中的破伤风抗毒素，以此替代目前公认的小鼠毒素中和试验。结果发现，ELISA操作简单、快速，仅需少量材料。此外，该测定法对抗毒素水平的估计具有可重复性，且能检测低至每毫升0.01国际单位的抗毒素，其灵敏度与中和试验相似。此外，对80份人血清（包括抗毒素水平高和低的血清）进行的ELISA和中和试验结果比较表明，两种方法所测得的抗毒素水平高度一致。得出的结论是，ELISA可作为小鼠毒素中和试验的可接受替代方法，用于检测人血清中的破伤风抗毒素水平。
