Fujiwara K, Asada H, Kitagawa T, Yamamoto K, Ito T, Tsuchiya R, Sohda M, Nakamura N, Hara K, Tomonaga Y, Ichimaru M, Takahashi S
J Immunol Methods. 1983 Jul 15;61(2):217-26. doi: 10.1016/0022-1759(83)90165-5.
An enzyme immunoassay for polyamines is described which uses beta-galactosidase labeled spermine and antiserum raised in rabbits against spermine-bovine serum albumin synthesized by coupling spermine to mercaptosuccinylated bovine serum albumin with a bifunctional cross-linker, N-(gamma-maleimidobutyryloxy)-succinimide. The lower limit of detection by this assay, which involves a double antibody technique for the separation of antibody-bound and free antigen, was 1 ng of spermine per tube. The anti-spermine serum showed 88% cross-reaction with spermidine but only 0.13% with putrescine, 0.08% with 1,3-diaminopropane, and 0.04% with cadaverine. The method has been used to measure serum polyamine levels in healthy subjects and cancer patients, in whom mean concentrations of 58.1 ng/ml and 94.8 ng/ml (as spermine), were respectively noted. This enzyme immunoassay is specific, accurate and easy to perform, and appears suitable for routine clinical use.
本文描述了一种用于多胺的酶免疫测定法，该方法使用β-半乳糖苷酶标记的精胺以及在兔子体内产生的抗血清，该抗血清针对通过双功能交联剂N-(γ-马来酰亚胺丁酰氧基)-琥珀酰亚胺将精胺与巯基琥珀酰化牛血清白蛋白偶联而合成的精胺-牛血清白蛋白。该测定法采用双抗体技术分离抗体结合抗原和游离抗原，其检测下限为每管1 ng精胺。抗精胺血清与亚精胺的交叉反应率为88%，但与腐胺的交叉反应率仅为0.13%，与1,3-二氨基丙烷的交叉反应率为0.08%，与尸胺的交叉反应率为0.04%。该方法已用于测量健康受试者和癌症患者的血清多胺水平，其中分别记录到平均浓度为58.1 ng/ml和94.8 ng/ml（以精胺计）。这种酶免疫测定法具有特异性、准确性且易于操作，似乎适用于常规临床应用。
