霍乱毒素操纵子内的核苷酸序列。
Nucleotide sequences within the cholera toxin operon.
作者信息
Gennaro M L, Greenaway P J
出版信息
Nucleic Acids Res. 1983 Jun 25;11(12):3855-61. doi: 10.1093/nar/11.12.3855.
Abstract
Nucleotide sequences coding for the N- and C-terminus of the A subunit and the N-terminus of the B subunit of cholera toxin were determined. These results show that the genes for the A and B subunits overlap out of phase by one nucleotide and that each subunit is synthesised as a precursor molecule which is subsequently processes after translation. It is proposed that the synthesis of each subunit is regulated at the translational level. Considerable homology with the heat labile toxin genes of enteropathogenic E.coli was noted.
摘要
测定了编码霍乱毒素A亚基的N端和C端以及B亚基的N端的核苷酸序列。这些结果表明，A亚基和B亚基的基因以一种核苷酸的相位错配方式重叠，并且每个亚基都是作为前体分子合成的，随后在翻译后进行加工。有人提出，每个亚基的合成在翻译水平上受到调控。还注意到与肠致病性大肠杆菌的不耐热毒素基因有相当的同源性。
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