Wakano K, Kasman R, Chao E Y, Bradford D S, Oegema T R
Spine (Phila Pa 1976). 1983 Jan-Feb;8(1):59-68. doi: 10.1097/00007632-198301000-00011.
With the renewed interest in using chymopapain (CP) as a chemonucleolytic agent for treatment of sciatica and low-back pain, the present study was undertaken to investigate the biomechanical property changes in canine lumbar discs after CP injections. The short-term (30- to 120-minute) in vitro effects of such an enzymatic agent appear to be the same as those of saline solution, causing increased disc heights, stiffness values, and creep rates. In the in vivo study, after three weeks, CP-injected discs had significant reductions in disc height and compressive stiffness, but the creep rate was increased substantially. However, at three months after injection, these biomechanical properties began to reverse and approached those of the uninjected controls. Buffer solution (cysteine and EDTA) was tried, but the sample size was too small to provide conclusive information. The results suggest that CP causes a disc to change its material property, but such effects appear to be time-related.
随着对使用木瓜凝乳蛋白酶（CP）作为化学核溶解剂治疗坐骨神经痛和腰痛的兴趣再度兴起，本研究旨在探讨CP注射后犬腰椎间盘生物力学特性的变化。这种酶制剂的短期（30至120分钟）体外效应似乎与生理盐水相同，会导致椎间盘高度、刚度值和蠕变率增加。在体内研究中，三周后，注射CP的椎间盘高度和压缩刚度显著降低，但蠕变率大幅增加。然而，注射后三个月，这些生物力学特性开始逆转并接近未注射对照组的特性。尝试了缓冲溶液（半胱氨酸和乙二胺四乙酸），但样本量太小，无法提供确凿信息。结果表明，CP会使椎间盘改变其材料特性，但这种效应似乎与时间有关。
