7S RNA：大肠杆菌RNA加工酶核糖核酸酶E的单一位点底物。

7 S RNA: a single site substrate for the RNA processing enzyme ribonuclease E of Escherichia coli.

作者信息

Szeberényi J, Roy M K, Apirion D

出版信息

Biochim Biophys Acta. 1983 Aug 2;740(3):282-90. doi: 10.1016/0167-4781(83)90137-9.

Abstract

7 S RNA accumulates at non-permissive temperatures in an RNAase E strain containing the recombinant plasmid pJR3 delta which carries a single 5 S rRNA gene and expression sequences. 7 S RNA is a processing intermediate that contains the complete sequence of 5 S rRNA as well as a stem-and-loop structure encoded by the terminator of rrnD. 7 S RNA can be processed in vitro by RNAase E. Structural analysis of the products (5 S rRNA and the stem) of in vitro processing of 7 S RNA revealed that the cleavage site of RNAase E in 7 S RNA is 3 nucleotides downstream from the 3' end of the mature 5 S rRNA. The cleavage generates 3'-hydroxyl and 5'-phosphate termini.

摘要

在含有携带单个5S rRNA基因和表达序列的重组质粒pJR3δ的RNA酶E菌株中，7S RNA在非允许温度下积累。7S RNA是一种加工中间体，它包含5S rRNA的完整序列以及由rrnD终止子编码的茎环结构。7S RNA可在体外被RNA酶E加工。对7S RNA体外加工产物（5S rRNA和茎）的结构分析表明，RNA酶E在7S RNA中的切割位点位于成熟5S rRNA 3'端下游3个核苷酸处。切割产生3'-羟基和5'-磷酸末端。