Szeberényi J, Apirion D
Biochim Biophys Acta. 1984 Oct 5;783(1):15-25. doi: 10.1016/0167-4781(84)90073-3.
A recombinant plasmid containing the promoters, terminators and only the intact 5 S rRNA gene of rrnB is expressed efficiently in Escherichia coli cells. In strains containing a thermolabile RNAase E (rne) full-length transcripts of the rrnB region from the plasmid and a partially processed intermediate product accumulate at non-permissive temperatures. Upon addition of chloramphenicol two additional plasmid-specific RNA molecules appear. They are shorter than the full-length transcripts. These species contain the 3'-end region of the full-length transcripts. Even though the 5' ends of these RNAs were most likely produced by degradative enzymes these 5' ends are not ragged. All these plasmid-specific RNAs are specific substrates for the two endonucleolytic RNA processing enzymes, RNAase E and RNAase III.
一种重组质粒，其包含rrnB的启动子、终止子且仅含有完整的5S rRNA基因，该质粒在大肠杆菌细胞中能高效表达。在含有热不稳定核糖核酸酶E（rne）的菌株中，来自质粒的rrnB区域的全长转录本以及部分加工的中间产物在非允许温度下会积累。加入氯霉素后会出现另外两种质粒特异性RNA分子。它们比全长转录本短。这些分子包含全长转录本的3'端区域。尽管这些RNA的5'端很可能是由降解酶产生的，但这些5'端并不参差不齐。所有这些质粒特异性RNA都是两种内切核酸RNA加工酶——核糖核酸酶E和核糖核酸酶III的特异性底物。
