rne基因是大肠杆菌加工内切核糖核酸酶RNase E的结构基因。
The rne gene is the structural gene for the processing endoribonuclease RNase E of Escherichia coli.
作者信息
Chauhan A K, Apirion D
机构信息
Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110.
出版信息
Mol Gen Genet. 1991 Aug;228(1-2):49-54. doi: 10.1007/BF00282446.
PMID:1715977
Abstract
Using T7 RNA polymerase and specific constructs derived from 5S rRNA and RNA I genes, we generated substrates for the RNA processing enzyme RNase E. Using these substrates we have shown that a 3.2 kb DNA fragment that complements the rne-3071 mutation can express RNase E activity. We also found that T7 RNA polymerase terminates within the 5S rRNA gene.
摘要
利用T7 RNA聚合酶以及源自5S rRNA和RNA I基因的特定构建体，我们制备了RNA加工酶核糖核酸酶E的底物。使用这些底物，我们已证明一个可弥补rne - 3071突变的3.2 kb DNA片段能够表达核糖核酸酶E的活性。我们还发现T7 RNA聚合酶在5S rRNA基因内部终止。
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