Wells C L, Balish E
Infect Immun. 1983 Aug;41(2):826-8. doi: 10.1128/iai.41.2.826-828.1983.
Germfree rats were challenged orally and intrarectally with spores of Clostridium tetani. Although C. tetani spores remained viable in the intestinal tract, they were unable to germinate. Germfree rats were then challenged orally with vegetative cells of C. tetani. Vegetative cells were able to colonize the intestinal tract, replicate, and produce toxin. Tetanus antitoxin, but no tetanus toxin, was detected in the sera of monoassociated rats.
无菌大鼠经口服和直肠内接种破伤风梭菌孢子。虽然破伤风梭菌孢子在肠道内仍保持活力,但无法发芽。然后给无菌大鼠口服破伤风梭菌的繁殖体。繁殖体能够在肠道内定殖、繁殖并产生毒素。在单菌定植大鼠的血清中检测到破伤风抗毒素,但未检测到破伤风毒素。