Korovina V P, Chikishev E V, Vaĭsman I Sh
Zh Mikrobiol Epidemiol Immunobiol. 1976 Oct(10):102-6.
A study was made of the dynamics of the population quantity, the delicate morphology of the bacterial cells and the toxin formation in the Cl. tetani 471 cultures grown in fluid nutrient medium with caseine hydrolysate in the industrial 100-litre reactors and under laboratory conditions. Endogenous metabolites contained in the filtrates of the periodic Cl. tetani 471 cultures which reached the phase of accelerated death were capable of enhancement of the toxin formation when introduced in the amount of up to 1 : 100 at the beginning of the exponential growth of the same cultures in the reactors. The growth of the some oligosporogenic strain in the filtrates of the periodic cultures obtained at the beginning of the stationary phase or of the accelerated death phase was characterized by a slower increase in the vegetative cell count, by enhanced spore-formation and by the second, after the initial, increase in the concentration of the microbes partially connected with the spore vegetation.
对破伤风梭菌471在工业用100升反应器和实验室条件下，于含有酪蛋白水解物的液体营养培养基中培养时的种群数量动态、细菌细胞的精细形态以及毒素形成进行了研究。处于加速死亡阶段的破伤风梭菌471定期培养物滤液中所含的内源性代谢产物，当在反应器中相同培养物指数生长开始时以高达1:100的量引入时，能够增强毒素形成。在稳定期开始或加速死亡阶段获得的定期培养物滤液中，某些少孢子形成菌株的生长特征为营养细胞数量增加较慢、孢子形成增强以及在初始之后微生物浓度的第二次增加，这部分与孢子萌发有关。
