Mossel D A, Bonants-Van Laarhoven T M, Ligtenberg-Merkus A M, Werdler M E
J Appl Bacteriol. 1983 Jun;54(3):313-27. doi: 10.1111/j.1365-2672.1983.tb02623.x.
To facilitate monitoring of culture media, a simple quantitative streaking technique, implying ever-decreasing numbers of colony-forming units per surface area, as in spiral plating, was developed. The procedure evaluates, in quantitative terms, the ability of media (1) to support the formation of colonies by organisms that it was designed to grow and (2) to resist colonization by organisms that it is expected to suppress. The procedure was therefore termed ecometric evaluation. The ecometric results appeared to agree well with observations made on productivity and selectivity of the media studied during routine examination of specimens. These encouraging results prompted further, rigorous standardization of ecometry. A template was developed to standardize inoculation and the depth of the agar was controlled to within +/- 10%. Finally the attributes of the inocula used were accurately defined. The standardized ecometric technique has been found useful for the following purposes: (1) to assess the practical significance of the inhibitory effect of gentamicin observed in some moulds and yeasts (this was solved by replacing poorer basal media by one particular richer modification, viz, yeast morphology agar); (2) the development of a blood-free selective enumeration medium for Campylobacter jejuni, i.e. sulphide iron motility agar plus the combination of antibiotics suggested by Skirrow (1977); and (3) verification of the absence of antimicrobial activity of enzyme preparations, e.g. catalase, used in culture media to remedy sublethal damage in certain groups of bacteria. Ecometric evaluation can now be recommended for (1) routine monitoring of consignments of dehydrated or ready-to-use, purchased media; and (2) in-house checking of the functioning of medium preparation departments. Only occasionally is it necessary to use conventional counting techniques to confirm the results.
为便于监测培养基，开发了一种简单的定量划线技术，如同螺旋平板接种法一样，意味着单位表面积上形成菌落的单位数量不断减少。该程序从数量上评估培养基的能力：（1）支持其设计用于培养的生物体形成菌落；（2）抵抗预期会被抑制的生物体的定殖。因此，该程序被称为生态计量评估。生态计量结果似乎与在标本常规检查期间对所研究培养基的生产力和选择性的观察结果非常吻合。这些令人鼓舞的结果促使对生态计量学进行进一步严格的标准化。开发了一个模板来规范接种，并将琼脂深度控制在±10%以内。最后，准确界定了所用接种物的属性。已发现标准化的生态计量技术可用于以下目的：（1）评估在某些霉菌和酵母菌中观察到的庆大霉素抑制作用的实际意义（通过用一种特定的更丰富的改良培养基，即酵母形态琼脂，取代较差的基础培养基来解决）；（2）开发用于空肠弯曲菌的无血选择性计数培养基，即硫化铁动力琼脂加斯基罗（1977年）建议的抗生素组合；（3）验证用于培养基以补救某些细菌亚致死损伤的酶制剂（如过氧化氢酶）是否不存在抗菌活性。现在可以推荐生态计量评估用于：（1）对脱水或即用型采购培养基批次的常规监测；（2）对培养基制备部门功能的内部检查。仅偶尔需要使用传统计数技术来确认结果。
